FastQCFastQC Report
Sun 14 Apr 2019
SRR6576179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295042
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6180.20946170375743112No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3760.12743948319222348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.11998291768629551No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3400.11523783054615953No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3160.10710339544878357No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3130.10608659106161157No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3110.10540872147016359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10600.041.2467081
TACATGG24050.036.554322
GTACATG24250.036.446711
ATGGGAG4750.035.6183555
ACATGGG24700.035.390033
AACGCAG12450.035.1057746
CATGGGT5800.034.842194
AGCTATA550.002714759234.179237
ATCAACG12950.033.750343
CATGGGG7850.032.927444
TCAACGC13500.032.723454
CAACGCA13750.031.7866825
ATGGGTA2750.030.7613055
ATGGGTG3250.030.366935
ATGGGCG953.7448663E-529.6819635
TATCAAC15100.029.2659842
ACGCAGA15100.028.9448267
CGCAGAG15600.028.0171098
CATGGGA12750.026.9077664
CTAGGAC909.5050613E-426.1091333
Produced by FastQC (version 0.11.2)