FastQCFastQC Report
Sun 14 Apr 2019
SRR6576178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309665
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6460.20861253289845477No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3880.12529669158606882No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3720.12012981770623092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.1143170845914133No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3470.11205657726898423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGT250.005238418556.3837248
GTATCAA9650.040.9133261
AGGCGGT500.001702519637.589157
GTACATG21050.034.832551
GTATTAC550.002713499634.1829951
ACATGGG21200.033.688393
TACATGG21800.032.987372
AACGCAG12050.031.9741736
GGCGGTT600.00416220731.3242918
TCAACGC12550.031.0746964
CAACGCA12600.030.5784765
ATCAACG12900.030.2315833
CATGGGG7400.029.2077854
CATGGGT5600.027.6884374
GTACCTT856.8162585E-427.639086
TTGTACC856.8162585E-427.639084
ACGCAGA14250.027.0378117
TATCAAC14450.026.9974692
CGCAGAG14550.026.480338
ATGGGTA3600.026.1035775
Produced by FastQC (version 0.11.2)