FastQCFastQC Report
Sun 14 Apr 2019
SRR6576174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440660
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11880.26959560659011483No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7860.17836881042073255No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7290.16543366768029774No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6410.14546362274769664No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6310.14319429945990106No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6230.1413788408296646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.13797485589797123No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5670.12867063041800936No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5300.12027413425316572No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5290.12004720192438614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5230.11868560795170881No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4960.11255843507466073No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4590.10416193890981709No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4420.10030408932056462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATG452.200486E-762.66477
GTATCAA12950.039.9805151
GATAATA1204.2000465E-935.2448962
GTACATG26650.034.9698261
TACATGG26850.034.6541982
TAATAGT1107.687777E-834.1768654
ACATGGG27950.032.954083
AACGCAG15950.032.1181146
ATCAACG16800.030.769353
GGTACCT4600.030.6720988
TCAACGC17000.030.4073584
GGTATCA7350.030.0978851
CATGGGG15950.029.7574444
GTTAAGG953.710299E-529.727141
CAACGCA17250.029.694255
GGGTACC4500.028.1991167
ATAGTAT1205.8338883E-627.4158066
GTACTAG700.00876819326.8959831
TGGGTAC4750.026.714956
TATAAGA909.516377E-426.1073272
Produced by FastQC (version 0.11.2)