FastQCFastQC Report
Sun 14 Apr 2019
SRR6576173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488618
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13730.2809966067561981No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8230.16843423697039406No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7870.16106651822077778No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7290.14919630467972936No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7160.14653573957570126No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6550.1340515494721848No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6210.12709314843088057No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5830.11931611197295228No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5360.10969714582761995No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5340.10928782811930793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.10744589843190386No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5090.10417135676540774No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5040.1031480624946277No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5000.10232942707800367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10200.052.083881
TACATGG26450.035.549162
GTACATG26750.035.3262331
TCAACGC15450.035.2875254
GGTATCA4400.034.1918331
ACGTCGT550.00271567534.1813357
ACATGGG27500.034.0104263
ATCAACG15900.033.9932333
AACGCAG16300.033.7357186
CAACGCA16450.033.1423875
GTATTAC753.2671908E-431.342511
GGTACCT5050.030.7124378
GGGTACC5300.030.1505177
TAGTATG804.7773024E-429.3745847
TGGGTAC5450.029.3206866
GTAGTGC650.006151177528.9226686
ACGCAGA19200.028.640227
GTATAAT1154.1803596E-628.6170771
CATGGGG16700.027.8618814
CGCAGAG20300.027.5513348
Produced by FastQC (version 0.11.2)