FastQCFastQC Report
Sun 14 Apr 2019
SRR6576172_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576172_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214588
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.15564710049024177No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3320.15471508192443192No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2270.10578410721941581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG405.6696683E-446.9944083
GTATCAA6550.035.9070661
CCTAGGT550.00271369234.177753
GTATAGC1151.12195266E-732.7222631
ACATGGG10100.031.63983
GTACATG10900.030.2080541
TACATGG10950.030.0420862
AACGCAG8650.029.337556
CATGGGC1951.8189894E-1128.9196364
CAACGCA8300.028.8760835
ATCAACG8350.028.703173
TCAACGC8350.028.703174
TCTACTC856.802636E-427.6437683
ACGCAGA9050.027.0023127
GTATTGG1057.353099E-526.8790041
TAGACTG700.00882542226.8539495
TCCTAGG700.00882542226.8539492
CGCAGAG9300.026.2948258
CATGGGG4850.026.1618354
GTGTAGC1807.36145E-926.1323621
Produced by FastQC (version 0.11.2)