FastQCFastQC Report
Sun 14 Apr 2019
SRR6576172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214588
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2960.13793874773985498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.1360747106082353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8300.040.2126961
CCTATAC550.00271570534.172583
ATCAACG9850.032.9149633
AACGCAG9700.032.4551476
CAACGCA9800.032.123975
TCAACGC10100.032.1002354
CAAACGC600.004149526431.3394762
TAGACAA753.2719184E-431.3248655
TATCAAC11300.028.7047392
ACATGGG14150.028.5576533
ACGCAGA11400.028.0275127
CATGGGG5600.027.6889444
CGCAGAG11550.027.6635198
GTACATG14450.027.3271521
TACATGG14400.027.0955872
TCCTATA700.00881187226.8624062
GGCTCGA700.00881187226.8624061
ATATACG1755.515176E-926.8498866
AAACGCA700.00883193726.8498843
TAATGCG350.008841610526.84988418-19
Produced by FastQC (version 0.11.2)