FastQCFastQC Report
Sun 14 Apr 2019
SRR6576171_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576171_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235972
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.17247809062092112No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2970.12586239045310463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG250.005233420556.393134
TATACCC250.005233420556.393134
GTATCAA7550.038.065681
TATAGCC906.1684113E-736.5511054
GTACTAG550.002680587134.264791
GACATAT600.00410532131.4093911
TGTTGCG300.004160997531.3295212-13
CATGGGG5600.029.3714244
ACATGGG13450.028.6507873
TACATGG14000.028.1965662
GTACATG14650.027.6574131
TAACCGG350.00883608526.85387416-17
ATATAAC700.00882729426.8538743
AACGCAG9900.026.5826236
ATCAACG9600.026.4342823
TCAACGC9850.025.7633634
CAACGCA10050.025.7182625
ATGGGAC1701.2365854E-724.8793245
TATCAAC11100.024.555572
ATGGGTA1602.029541E-623.4971395
Produced by FastQC (version 0.11.2)