FastQCFastQC Report
Sun 14 Apr 2019
SRR6576169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383278
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.16906788284221896No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6040.15758796487145102No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3860.10071018947082798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17400.039.1663971
AACGCAG20150.034.2786566
ATCAACG20750.033.061023
CAACGCA20850.032.9024545
TCAACGC20850.032.9024544
GTATTAC600.00415769431.3331151
ACGCAGA22900.030.1622267
TATCAAC23100.030.1123432
CTAATGC953.7526785E-529.676263
ATGGGAG5250.029.5349455
CGCAGAG23550.029.1301988
GTACATG28900.028.4600091
TACATGG29400.027.9759962
ACATGGG29550.027.5087053
GCAGAGT26000.026.3852379
TACGCTA2651.8189894E-1224.8235369
GGTCTAG1151.3749345E-424.5215681
ATGGGCG1151.3773727E-424.515175
TATACTG1751.6444756E-724.1649535
CATGGGC4900.023.973174
Produced by FastQC (version 0.11.2)