FastQCFastQC Report
Sun 14 Apr 2019
SRR6576167_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576167_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559581
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.12044726322015936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.037.4885181
TATACCG550.002717747434.176885
AACGCAG19800.030.8541246
GTACATG30950.029.807781
TCAACGC20500.029.8005714
CAACGCA20550.029.7280655
ATCAACG20750.029.2150553
TACATGG31400.028.8869422
ACATGGG30950.028.6969853
CATGGGG15000.027.8826374
TATCAAC22250.027.2479362
ACGCAGA22700.027.1194297
ATAGGCC909.5195783E-426.1073383
CGCAGAG24250.025.4041848
GTACTAG1000.001742632623.5344581
GTGTTAG1201.8182535E-423.5344561
GATAACG601.8398048E-423.49660590-91
GCAGAGT27350.022.8686229
ATGGGGA8500.021.0087285
GTATTAG1605.345833E-520.5926511
Produced by FastQC (version 0.11.2)