FastQCFastQC Report
Sun 14 Apr 2019
SRR6576165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400850
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6780.16914057627541473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.15292503430210802No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4380.10926780591243607No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4360.10876886615941124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11800.050.6594431
CACGTAC450.001005833241.839471
ATCAACG15100.039.212773
TCAACGC15100.039.212774
AACGCAG15550.038.6824046
CAACGCA15700.038.0135085
TATCAAC17050.035.003642
GTATAAG1107.576091E-834.2322921
ACGCAGA17750.033.6232157
CGCAGAG17950.033.5312968
GTACATG23350.030.4388831
TACATGG23300.030.2530542
ACATGGG23050.029.765683
GCAGAGT21000.028.6612769
GGTCGCG700.00883562626.853197
TGCACCG700.00883562626.853195
CATGGGG10000.026.3161224
GGTATCA5600.024.3752271
CATGGGT5700.023.9087584
TCTACTG1401.929449E-523.496545
Produced by FastQC (version 0.11.2)