FastQCFastQC Report
Sun 14 Apr 2019
SRR6576164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432182
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6490.1501682161681884No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4810.1112957041246512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.10296588011532179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.057.130381
ATCAACG12750.040.5466773
TCAACGC13250.039.0166134
CAACGCA13400.038.5798575
AACGCAG14050.036.7950256
TATCAAC15100.034.551672
TACATGG25300.032.511762
ACATGGG25550.032.373863
GTACATG25750.031.9435961
CGCAGAG16450.031.4267548
ACGCAGA16700.030.9562937
GAACCGG804.7776225E-429.37336
CATGGGT6650.028.9757674
CATGGGA12050.026.9113084
ATGGGAG6800.026.2631865
GCAGAGT19800.026.10969
GGTATCA3650.025.7549171
CATGGGG10400.024.8543324
AGAGTAC17850.023.95939810-11
ATGGGTA3800.023.498645
Produced by FastQC (version 0.11.2)