FastQCFastQC Report
Sun 14 Apr 2019
SRR6576163_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576163_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410055
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6940.1692455890063528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.1597346697394252No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4900.11949616514857764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4650.11339942202875224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGT250.00523641456.3924646
GTATCAA11550.048.9440231
AACGCAG16050.035.135496
ATCAACG16100.035.0263753
TCAACGC16350.034.203384
CAACGCA16600.033.6882675
TACATGG24950.033.1498762
GTACATG25500.032.8836631
TATCAAC17500.032.2242662
ACATGGG25650.032.0619933
TATGCCC600.0041606131.3291455
GGTATCA5700.029.7528131
ACGCAGA19300.029.2188937
CGCAGAG19500.029.1779968
CATGGGA11850.028.949724
GCAGAGT21150.026.90179
CATGGGG9800.026.8535564
GTAATAG950.001285736424.794011
ATGGGAG5600.023.496865
CATGGGT6900.023.1563264
Produced by FastQC (version 0.11.2)