FastQCFastQC Report
Sun 14 Apr 2019
SRR6576160_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576160_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199784
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5720.2863092139510671No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4370.21873623513394466No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4280.2142313698794698No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3840.1922075841909262No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3470.17368758258919634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.1596724462419413No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2940.14715893164617788No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2740.13714811996956713No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2730.1366475793857366No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2570.128638930044448No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2520.12613622712529532No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2460.12313298362231209No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2410.12063028070315941No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2360.11812757778400672No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2330.1166259560325151No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2240.11212109077804028No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2220.11112000961037921No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2210.11061946902654868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2050.10261081968526008No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2040.10211027910142954No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2030.10160973851759901No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2030.10160973851759901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG301.3686728E-462.6641588
TGATCGC352.9322057E-453.7121359
CTATTAT1308.89122E-932.6188131
CATGGGG6400.031.584774
CATACTG753.261907E-431.3399285
TGGGTAC2150.030.611096
GTACATG15750.029.6158081
ACATGGG15750.029.5490723
TACATGG15500.029.4190922
GTATCAA9650.028.8067111
TATTATC1154.1667827E-628.6147162
ATGGGTA2151.8189894E-1228.4245835
CATGGGT3200.027.9121234
ATGGGGA3500.026.8627955
ATGGGGG2104.7293724E-1126.8627935
ATACTGT1057.380861E-526.8627936
ATAAGGT700.008809824526.8627933
CCAGTCC350.00883096926.85606836-37
AACGCAG10950.026.6174726
GTACAAA909.3543594E-426.175591
Produced by FastQC (version 0.11.2)