FastQCFastQC Report
Sun 14 Apr 2019
SRR6576159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519113
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9690.1866645605099468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.1385054891709512No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5500.10594995694579022No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5270.10151932238260263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11800.037.536851
TCAACGC13800.031.3290944
GTACATG22400.031.1333351
ATCAACG13900.030.7656233
CAACGCA13950.030.6553525
TACATGG22750.030.5805242
AACGCAG14650.029.5113686
ACATGGG23350.028.7798333
GGTATCA6650.028.3432941
TACACCG856.816406E-427.643325
CATGGGG12000.027.0213454
TATCAAC17400.025.3947092
ACGCAGA17450.024.237417
CGCAGAG17600.024.030848
CATGGGT6450.023.3146744
ATTACAC2103.850255E-822.3779263
GGACCGT1050.00233561922.3779266
ATGGGGA7150.022.3466285
GACCGTG1703.4498426E-622.1146567
GCTATAC1703.4498426E-622.1146563
Produced by FastQC (version 0.11.2)