FastQCFastQC Report
Sun 14 Apr 2019
SRR6576158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469168
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7720.16454660164376086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.16284145551273743No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4750.10124305152951608No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4740.10102990826313814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12100.047.5374681
ATCAACG15550.035.0589073
AACGCAG16000.034.3666086
CAACGCA16100.033.8612445
TCAACGC16150.033.756414
GTACATG23550.032.432891
TACATGG24450.031.338062
ACATGGG23900.031.069193
ACGCAGA18000.030.5480947
TATCAAC18400.030.1458512
CGCAGAG18800.029.2481778
CATGGGG10250.028.8860034
GTACTTG2700.027.9394451
CATGGGT5650.025.7860034
GCAGAGT21800.025.6543649
AGAGTAC20200.023.26587510-11
GGGTACC3900.022.8960087
GTATTAT1252.369551E-422.630951
ATGGGTA3550.022.5056385
GGTACCT3550.022.5056388
Produced by FastQC (version 0.11.2)