FastQCFastQC Report
Sun 14 Apr 2019
SRR6576157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325408
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5330.1637943750614613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.10909381453436916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC250.00523843656.3843277
GTATCAA10700.036.8926121
TAACACC1001.410106E-632.8908584
GTACATG21200.031.0338781
TACATGG21700.030.96852
AACGCAG12800.030.101016
GGTCTAA1102.9627008E-629.9053751
ACATGGG21900.029.3936563
ATCAACG13400.029.1038513
CAACGCA13300.028.969395
TCAACGC13550.028.7816684
TATCAAC14250.027.701822
CTATGTA1205.838925E-627.409054
ACCGGCA350.00880553626.87445688-89
CATGGGG8250.026.7683184
ACGCAGA14500.026.5719247
CGCAGAG15050.025.6008578
CTAAGCT950.001304688924.7299694
ATAACAC950.001304688924.7299693
ACCATAT1151.3768415E-424.5149258
Produced by FastQC (version 0.11.2)