FastQCFastQC Report
Sun 14 Apr 2019
SRR6576156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358215
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5450.152143265915721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10300.038.8125151
AACGCAG12000.034.0741426
GTACATG20700.032.2632521
CAACGCA12750.031.7011625
ATCAACG12900.030.9682123
ACATGGG21450.030.6752153
TACATGG21550.030.5541952
TCAACGC13000.030.3684654
TATCAAC14200.028.483952
ACGCAGA14800.027.6276827
CGCAGAG14900.027.4422598
CATGGGT4150.027.1800384
ATTGGGC700.00882913926.8564643
ATGGGAG5950.025.2766725
GTATAGA950.001297732824.7534921
CATGGGA12050.024.5719934
GCAGAGT17150.024.3900559
GTATATA2151.8535502E-924.0626981
GGTATCA6150.023.7070051
GTCTATA1201.8262575E-423.515821
Produced by FastQC (version 0.11.2)