FastQCFastQC Report
Sun 14 Apr 2019
SRR6576155_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576155_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292858
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.1516093123629882No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3770.1287313305424472No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3190.10892651045899376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7650.038.776681
TCTAGAC902.5913336E-531.3263343
ACATGGG15850.030.8322263
GTACATG16000.030.605881
CTATAAG1102.9166367E-629.9637991
TACATGG16200.029.881142
ATCAACG9900.029.427773
AACGCAG10050.028.9885486
GTAATAG650.006092129728.9759851
CAACGCA10300.028.2849435
TAGTACT1005.3182783E-528.19374
TCAACGC10350.028.1482984
GGCAATA856.731547E-427.697631
ATAGACT856.812773E-427.640884
TCTAACG350.00880175326.8763870-71
CATGGGT3700.026.6697184
CATGGGA8600.026.22674
GGGTAGC909.399845E-426.1588731
GGTTAGC909.475218E-426.1231178
GTATAAT2357.2759576E-1226.0475581
Produced by FastQC (version 0.11.2)