FastQCFastQC Report
Sun 14 Apr 2019
SRR6576155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292858
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4020.1372678909232461No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.13351180435569457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9100.046.008411
ATCAACG10800.038.2914963
CAACGCA11150.037.0831875
AACGCAG11150.037.0831876
TCAACGC11250.036.759844
CATGGGT3200.035.2455834
CCTAATA550.00270176834.212592
TACATGG15400.034.212592
ACATGGG15550.033.5456353
TATCAAC12350.033.5200232
CGCAGAG12350.033.4799658
GTACATG15750.033.452311
ACGCAGA12550.032.946427
ATCACGT600.00416199331.32405794
GCAGAGT13900.029.7465869
ATGGGCG953.7504084E-529.6754255
GATCGTC1005.3209133E-528.191657
GCATAGG856.7681685E-427.6719441
ATGGGTA2600.027.1073555
CCCTATA700.008786811526.8813192
Produced by FastQC (version 0.11.2)