FastQCFastQC Report
Sun 14 Apr 2019
SRR6576154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321859
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4090.1270742778670163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.12396732730792055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.050.76391
ATCAACG11100.036.4141733
TCAACGC11300.035.7696764
AACGCAG11500.035.556296
CAACGCA11600.034.84465
TATCAAC12950.032.310982
GGTATCA3350.032.2784731
TACATGG17350.030.078312
ACATGGG17400.029.7124463
GTACATG17900.029.6794171
CGCAGAG14050.029.103018
ACGCAGA13900.029.0789457
TAGTAGT1205.826363E-627.4164854
GCAGAGT16350.025.0090089
CATGGGG6250.024.8158344
ATAGTAG950.00130259724.7366773
GTATAAG1151.371367E-424.5291981
GTATAGG1201.8302677E-423.5071491
TATAAGA1201.8302677E-423.5071492
GTATATG1050.002327754922.387761
Produced by FastQC (version 0.11.2)