FastQCFastQC Report
Sun 14 Apr 2019
SRR6576152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147469
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4490.3044707701279591No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2990.20275447721215983No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2830.19190473930114127No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2280.15460876523201486No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2240.1518963307542602No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2110.14308091870155762No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2080.14104659284324164No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2010.136299832507171No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1910.12951874631278437No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1830.12409387735727508No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG1810.12273766011839776No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1770.12002522564064312No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1770.12002522564064312No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1750.11866900840176578No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1730.11731279116288848No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1720.1166346825434498No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1700.11527846530457249No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1700.11527846530457249No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG1630.11053170496850184No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1610.10917548772962453No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG1600.10849737911018587No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1570.10646305325186989No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1570.10646305325186989No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.10510683601299257No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG1490.10103818429636059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG250.00522716756.3995975
GTGCAAG451.6038824E-552.239561
TGCAAGG502.9907049E-547.0156062
TGCCCGC853.9142833E-738.7187351
TCTATTC757.3999745E-637.5997288
GTATCAA4700.035.011621
GACAATG959.377145E-734.631337
GGACAAT959.377145E-734.631336
GTACATG10700.033.8336561
CTATTCA851.7445624E-533.176239
ACATGGG11050.032.3255583
TACATGG11350.032.3102842
CATGGGC1658.54925E-1131.3331094
CACTCTA1052.049388E-631.3331079
AACGCAG5250.031.3331056
TGGGTAC1401.8675564E-830.214076
CATGGGT2650.030.1507244
ATGGGTA1602.1009328E-929.3747885
CAACGCA5800.029.1722035
AAGTAGT650.006137239728.9228696
Produced by FastQC (version 0.11.2)