FastQCFastQC Report
Sun 14 Apr 2019
SRR6576147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344127
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8080.23479703714035807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.14471401546522067No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4350.12640682073769277No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4030.1171079281776786No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3660.10635608365516219No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3600.10461254130015954No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3450.1002536854126529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG250.00518976256.518151
GTCTTAT651.3941599E-436.229581
GTATCAA11050.034.5246621
TAAGAGT851.754045E-533.1686744
GTATAGG600.004114598531.3989721
ACGCGTT300.00415869831.33507768-69
CATGGGT4550.030.9817314
GTACATG19900.030.0578081
TACATGG19950.029.6858632
ACATGGG20600.029.4251233
GGGTACC2900.029.165567
CACGCGG650.006146586528.92468594
TCTAGGG650.00615532328.9162793
TCAACGC12800.028.2667944
CAACGCA12950.027.939385
ATCAACG13000.027.831923
AACGCAG13400.027.351786
GTACTTG2800.026.9134041
TATAGGG700.00882517726.8586372
ATACGAG350.00883120426.85863754-55
Produced by FastQC (version 0.11.2)