FastQCFastQC Report
Sun 14 Apr 2019
SRR6576146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831611
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19370.23292140195355762No Hit
GCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCC13560.16305700622045644No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12690.15259538413994045No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT12670.15235488708061823No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11160.1341973591017916No Hit
GTACATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC11130.13383661351280826No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11060.13299487380518055No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10930.1314316429195862No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10500.12626095614415875No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10440.12553946496619212No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10150.12205225760602012No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC10060.12097002083907019No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9460.11375510905940397No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8740.10509721492380451No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8720.10485671786448231No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8570.10305298991956577No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG8550.10281249286024355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17450.064.4637151
GGTATCA5750.059.7541241
ATCAACG25950.042.7373923
TCAACGC26300.042.526014
CAACGCA26800.041.907965
AACGCAG27900.040.2556726
TATCAAC32300.034.7719272
ACGCAGA34600.032.4605037
CGCAGAG35000.031.9764068
GCGCGAC4100.029.8201779
CCTTACG953.7548412E-529.6798082
GTACATG42000.029.5847231
ACGCGCG2550.029.5106694
GTCTTAG3550.029.1680341
TACATGG42050.028.9445742
TACGGTA1154.1994044E-628.604455
ACATGGG42150.028.3184533
GCAGAGT39450.028.2502359
TTACGGT1005.3271728E-528.1958144
ACGGTAC1005.3271728E-528.1958146
Produced by FastQC (version 0.11.2)