FastQCFastQC Report
Sun 14 Apr 2019
SRR6576129_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576129_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068494
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16450.15395500583063637No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG13200.12353836334130094No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12900.12073067326536227No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12810.11988836624258069No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12200.1141793964215054No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11330.10603709520128331No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG11060.1035101741329385No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11000.10294863611775079No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT10820.10126402207218758No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10770.10079607372619781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20900.063.7469141
GGTATCA6250.055.7404821
ATCAACG29000.044.8856353
TCAACGC29200.044.7391324
CAACGCA29450.044.3593445
AACGCAG30600.042.9993866
TATCAAC35300.037.5422552
ACGCAGA37150.035.166737
CTTAGGT2650.033.6925133
CGCAGAG40150.032.679068
GCAGAGT40850.032.23429
TTAGGTA3100.030.3175374
GTACATG47650.027.1699471
GTCTTAG4550.026.9017771
TAGGTAT3350.026.6522835
TACATGG48450.026.5768052
GTATTAC1257.920422E-626.363741
AGAGTAC37300.026.16135410-11
AGGTATA3250.026.026446
ACATGGG48900.025.9466043
Produced by FastQC (version 0.11.2)