FastQCFastQC Report
Sun 14 Apr 2019
SRR6576129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068494
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16520.15461013351502206No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13280.12428708069488457No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12560.1175486245126318No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12480.11679990715904814No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12270.1148345241058911No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT11650.10903196461561787No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11600.10856401626962808No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11480.10744094023925263No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10980.10276145677935487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19600.061.3901981
GGTATCA5800.060.7782821
ATCAACG27750.043.0074423
TCAACGC27850.042.8530164
CAACGCA28900.041.296075
AACGCAG29300.040.8926666
TATCAAC34450.034.65452
ACGCAGA35900.033.1130337
GTACATG48500.031.6899221
TACATGG49600.030.8923592
ACATGGG50350.030.4222283
CGCAGAG39250.030.2868238
GCAGAGT39950.029.991379
CCCTATA856.8141846E-427.648162
CTTAGGT3200.026.4298923
CGTATAG1258.020739E-626.3210471
TTAGGTA3400.026.2571474
AGAGTAC36000.026.23411610-11
TAGGTAT3300.025.6289865
GGTACCT8100.025.5235188
Produced by FastQC (version 0.11.2)