FastQCFastQC Report
Sun 14 Apr 2019
SRR6576126_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576126_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084354
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11950.11020386331401001No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10920.10070512028359743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16350.057.3418271
GGTATCA5300.048.890341
TCAACGC22150.042.0150684
ATCAACG22650.040.6744353
CAACGCA23250.039.8232655
TAAACGT608.821866E-539.1680874
AACGCAG24650.037.9428336
TATCAAC26000.035.9760442
ACGCAGA30100.030.4468027
GTACATG41750.029.1138361
TACATGG41600.028.925462
CGCAGAG31750.028.568488
ACATGGG42950.027.9068413
GCAGAGT33750.026.7362829
TATAGGG1606.909795E-826.4396762
ACCTAAG4150.024.9753061
AGAGTAC30050.024.63256510-11
CTAAGAC7100.024.4949763
CTAGACG1000.00175777223.5019363
CATGGGG24900.022.8401854
Produced by FastQC (version 0.11.2)