FastQCFastQC Report
Sun 14 Apr 2019
SRR6576115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423750
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15290.10739244951712028No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14770.10374012291483757No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14470.10163301141352063No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14360.10086040386303775No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14310.10050921861281827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24600.061.7153971
GGTATCA8000.056.4036671
TCAACGC33850.044.290684
ATCAACG34600.043.194793
CAACGCA35600.042.3775025
AACGCAG36950.041.0835956
TATCAAC42800.035.5817532
ACGCAGA45550.033.3268667
CGCAGAG49400.030.634398
GCAGAGT52850.028.812469
GTACATG58650.028.5304131
TACATGG60300.027.282042
ACATGGG60700.026.9445483
ACCTAAG3350.026.658451
AGAGTAC45650.025.78975910-11
TAAGACA8300.022.6496894
CATGGGG35050.022.258734
TTGTACG1303.1610215E-421.6914334
AGTACAT40050.021.29876512-13
CTATTAA4450.021.1249681
Produced by FastQC (version 0.11.2)