FastQCFastQC Report
Sun 14 Apr 2019
SRR6576114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473459
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13560.2864028353035849No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9190.19410339649262132No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8170.1725598203857145No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8140.17192618579433488No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6740.14235657152995296No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6260.13221841806787918No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5800.1225026876667251No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5600.11827845705752768No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5540.11701118787476845No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5540.11701118787476845No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5480.11574391869200924No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5400.11405422644833027No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5350.11299816879603092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.10898514971729337No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5060.10687303441269465No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4990.10539455369947556No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4780.10095911155981827No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4750.10032547696843866No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4740.1001142654379788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.040.826561
GTACATG29900.035.8409961
TACATGG30550.034.924572
ACATGGG29950.034.8285143
GTATACC550.002719054234.172453
AACGCAG16200.033.6450966
ATAGAGC1001.4118632E-632.890983
TCAACGC16600.032.8343734
GGTACCT4950.032.273988
CAACGCA17050.032.243365
ATCAACG17100.031.8743023
CATGGGT8350.030.3868774
ATGGGTA5250.029.5347585
CATGGGG12500.029.319964
TGGGTAC5350.028.9827066
ACGCAGA19950.027.320837
GGGTACC5700.027.2030667
CGCAGAG20200.026.98278
CATATAC1258.027962E-626.3127843
GTATTAA2008.094503E-1025.8511051
Produced by FastQC (version 0.11.2)