FastQCFastQC Report
Sun 14 Apr 2019
SRR6576111_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576111_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172509
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19000.16204566446824717No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14850.12665147986070896No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14430.12306941780404244No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13010.11095863656483661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20300.063.7453271
GGTATCA6100.060.1695061
ATCAACG27100.047.858433
TCAACGC27300.047.6799474
CAACGCA28200.046.491525
AACGCAG29200.045.0602726
TATCAAC32300.040.1553762
ACGCAGA36250.036.296837
CGCAGAG37900.034.617738
GCAGAGT41500.031.8413739
GTACATG50350.031.1212081
TACATGG52750.029.4878222
ACCTAAG6650.029.0117471
ACATGGG52750.028.8629823
TAGGTAT4900.028.770265
CTAAGAC8550.027.4803683
TTAGGTA5500.027.3404674
GTCTTAG6500.026.785521
CTTAGGT5400.026.106353
CATGGGG27300.025.6473354
Produced by FastQC (version 0.11.2)