FastQCFastQC Report
Sun 14 Apr 2019
SRR6576110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314832
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18750.14260376991128904No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18110.13773622789831705No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15600.11864633656619247No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14580.11088869148301837No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14290.10868308650839043No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14010.10655353687771517No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13960.10617326015795174No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13680.10404371052727648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8050.064.232991
GTATCAA24450.059.9842221
TCAACGC32000.045.530974
ATCAACG32450.044.8995743
CAACGCA32950.044.503525
AACGCAG34200.042.8769346
TATCAAC38300.038.0473332
ACGCAGA41450.035.6041267
CGCAGAG44750.032.6634868
GCAGAGT48200.030.7155849
GTACATG58050.029.7183821
TACATGG59400.028.7264162
ACATGGG61750.027.552873
CTTAGGT5150.026.465863
AGGTATA5150.025.5532446
AGAGTAC42150.025.42333210-11
GTCTTAG6550.025.1181721
TAGGTAT5250.025.0665155
TTAGGTA5650.024.1237474
CTAAGAC8900.023.2358153
Produced by FastQC (version 0.11.2)