FastQCFastQC Report
Sun 14 Apr 2019
SRR6576096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372970
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC6830.18312464809502105No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5450.14612435316513392No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4000.10724723168083222No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3890.10429793280960936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6400.055.0705031
GGTATCA2050.055.0167771
TCAACGC9200.038.815514
CGATAGG500.001701709637.5947951
CAACGCA9500.037.589765
ATCAACG9500.037.0951583
AACGCAG9650.037.005466
TATCAAC10200.034.554042
CTATATG550.002716508534.1770861
GTGTAAT550.002716508534.1770861
GGTAATC1107.691051E-834.1725048
GTACATG15450.031.9373281
ACATGGG16100.031.8112093
GTATAGG753.2739883E-431.3289991
TACATGG16200.030.748832
ACGCAGA11950.029.4898727
CGCAGAG12000.029.3669978
TATACAG804.7823857E-429.3669975
ACTGATC856.818248E-427.6395268
TAACACC856.818248E-427.6395269
Produced by FastQC (version 0.11.2)