FastQCFastQC Report
Sun 14 Apr 2019
SRR6576095_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576095_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479373
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9130.19045711794364723No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9040.1885796655214207No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6060.12641512976325325No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC5990.12495488899041038No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5730.11953113754842262No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5230.10910084631383077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7350.061.563471
GGTATCA2000.061.274891
GTTAACT405.672333E-447.00177494
TCAACGC10450.044.0782174
ATCAACG11000.041.8743063
CAACGCA11300.041.594495
AACGCAG11800.039.8320126
TATCAAC12150.037.91092
ACGCAGA13900.033.81077
GTATAGA702.146759E-433.6675261
CGCAGAG15200.030.9189938
GTACATG18200.030.041791
CTTAAGG953.7467984E-529.685333
TAAGACA4200.029.0963344
ACCTAAG3300.028.5663831
TACATGG19150.028.2256092
ACATGGG19000.027.9536863
CTAAGAC4400.027.7737733
GCAGAGT17000.027.3687659
ATTATCC4500.027.156583
Produced by FastQC (version 0.11.2)