FastQCFastQC Report
Sun 14 Apr 2019
SRR6576095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479373
Sequences flagged as poor quality0
Sequence length100
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8500.1773149509880615No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6990.1458154714595941No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC5180.1080578171903716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8500.056.9639431
GGTATCA2100.051.486131
TCAACGC11450.041.8683474
CAACGCA11950.040.509835
ATCAACG11650.040.342723
AACGCAG12400.039.797776
TATAGGG651.4106576E-436.1608282
GTAGTAC651.4123901E-436.1532864
TATCAAC13200.035.9690672
TAGTACT550.002715642334.181295
ATGCGTA352.184932E-433.57090884-85
ACCTAAG3700.033.0334051
TAATAGG1001.409062E-632.8994874
GTCTAGA600.00415465531.3393841
ACGCAGA15750.030.736037
CGCAGAG16050.029.5758658
TAAGACA5700.028.8592014
CTAAGAC5550.027.945513
CTATATT856.810872E-427.6466274
GCAGAGT17500.027.393869
Produced by FastQC (version 0.11.2)