FastQCFastQC Report
Sun 14 Apr 2019
SRR6576087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334776
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16460.12331657146966982No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16410.12294197678112283No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15820.1185217594562683No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15640.11717321857749916No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14520.10878229755404653No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13950.10451191810461082No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13680.10248910678645705No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13410.10046629546830331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20900.061.1818281
GGTATCA6850.046.6678921
ATCAACG27900.044.7970543
TCAACGC28000.044.6370664
CAACGCA28950.043.172295
AACGCAG30500.041.2863966
TATCAAC36400.034.2250672
ACGCAGA38750.032.4963877
GTACATG48700.031.7589971
CGCAGAG40150.031.1292118
ACATGGG49250.030.7200323
TACATGG49100.030.638562
ACCTAAG3700.027.952511
GCAGAGT45250.027.6207249
CATGGGA23200.026.1260494
TATAGGG1807.481503E-926.1172452
TAAGACA8900.025.340974
AGAGTAC38400.024.83915510-11
GCGTAGT1401.9281128E-523.5055181
GTCTTAG7800.022.9028111
Produced by FastQC (version 0.11.2)