FastQCFastQC Report
Sun 14 Apr 2019
SRR6576086_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576086_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499206
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17380.11592803123786857No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15330.10225412651763666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23100.056.9073371
GGTATCA8750.051.1554071
TCAACGC29350.044.2040144
ATCAACG30000.042.7790453
CAACGCA30800.041.8177385
AACGCAG31550.040.8236586
TATCAAC39200.033.0988352
GTCTTAG6750.032.1092451
ACGCAGA40500.031.795777
CGCAGAG42800.030.1979338
GTACATG54550.028.8487991
TACATGG54750.028.077172
TAGGTAT4200.027.9802425
ACATGGG55400.027.7477463
CTTAGGT4450.027.4664843
GCAGAGT48850.026.4579649
GTCTACG3600.024.8672121
AGGTATA4400.024.5717376
AGAGTAC41100.024.35712810-11
TACGTGT3850.023.1981624
Produced by FastQC (version 0.11.2)