FastQCFastQC Report
Sun 14 Apr 2019
SRR6576083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108199
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16410.1480780978867514No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16110.14537100286139945No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15600.14076894131830114No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14290.12894795970759765No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12540.1131565720597113No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11830.10674978049971169No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11740.10593765199210611No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11490.1036817394709795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22150.062.4021761
GGTATCA6650.057.2650261
ATCAACG28650.047.8883133
TCAACGC29050.047.228924
CAACGCA30850.044.4732635
AACGCAG32050.042.8081176
TATCAAC33250.041.9943542
ACGCAGA38900.035.149147
TAGGTAT7100.034.4125065
CTTAGGT6700.034.3631173
GTCTTAG8250.033.6220471
CGCAGAG41550.032.907388
AGGTATA7050.031.9906776
GGTATAG7000.031.5479477
GCAGAGT44600.030.8676859
TTAGGTA7450.029.6423684
CCGTATG804.788936E-429.3664429
TCTTAGG8850.029.2176672
GTACATG47650.029.2048451
GTATAGA1652.9540388E-928.4932651
Produced by FastQC (version 0.11.2)