FastQCFastQC Report
Sun 14 Apr 2019
SRR6576082_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576082_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235050
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18910.1531112100724667No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18020.1459050240880936No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14260.11546091251366342No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13880.11238411400348164No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13010.10533986478280231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9250.057.0232961
GTATCAA23350.054.8602871
TCAACGC31350.040.1784554
ATCAACG31500.040.137963
CAACGCA32600.038.49375
AACGCAG33900.037.4334686
TATCAAC36850.034.3106042
GTACATG56950.032.333971
TACATGG59000.030.9095942
ACGCAGA41650.030.2411447
ACATGGG59300.030.1983933
CGCAGAG44200.028.4964628
GGTAATC2151.8189894E-1228.4173328
TAAGCGT1057.4222306E-526.8570274
GTCTTAG6850.026.1257651
CATGGGG33350.026.0718524
GCAGAGT49800.025.3864149
CTAAGAC9100.024.7921053
TAAGACA10550.024.5022664
TAGGTAT5200.024.4037425
Produced by FastQC (version 0.11.2)