FastQCFastQC Report
Sun 14 Apr 2019
SRR6576081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621016
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9280.1494325428008296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.11304056578252412No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6290.10128563515271749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18000.044.9377481
ATCAACG22500.036.3447953
CAACGCA22800.036.072715
AACGCAG22950.035.8369376
TCAACGC23100.035.4007764
TACATGG39350.035.1364562
ACATGGG39450.034.905683
GTACATG40300.034.5415651
ACGCAGA26700.030.803667
TATCAAC26400.030.6393742
CGCAGAG27300.030.1266568
CATGGGG15700.029.0367224
GTATAAC1154.193984E-628.6072273
CATGGGA22200.027.5211074
GCAGAGT31800.026.0112429
TAATACC1351.4566189E-524.369124
GTATTAT2552.1827873E-1123.9749971
ATTATAC1401.9302306E-523.4987933
CATGGGT9000.023.498794
ATGGGAG10000.023.498795
Produced by FastQC (version 0.11.2)