FastQCFastQC Report
Sun 14 Apr 2019
SRR6576080_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576080_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515945
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18680.12322346786987654No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18520.1221680206076078No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15960.1052808644113078No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15280.1007952135466656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22100.059.8637731
GGTATCA8000.048.2585141
TCAACGC30000.043.5468064
ATCAACG30550.042.762823
CAACGCA31100.042.0065655
AACGCAG32500.040.341656
TATCAAC39050.033.4546552
TAGGTAT5150.031.0244775
ACGCAGA42850.030.5975177
CGCAGAG44100.029.9641158
GTCTTAG7850.029.3884371
GTACATG57050.029.1319241
TACATGG57350.028.4334032
AGGTATA4650.028.2968356
ACATGGG58150.028.2038563
CTTAGGT4750.027.7011133
GGTATAG5000.026.3160577
ACAACGC909.488955E-426.1252948
GCAGAGT50700.026.063469
ACCTAAG7500.025.7378751
Produced by FastQC (version 0.11.2)