FastQCFastQC Report
Sun 14 Apr 2019
SRR6576080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515945
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19110.1260599823872238No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16890.1114156516232449No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16760.11055810072265154No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15860.10462120987238983No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15580.10277417716341951No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15270.10072924809277381No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15250.10059731718499022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24950.058.3975751
GGTATCA8300.053.2296261
TCAACGC32500.043.661464
ATCAACG32750.043.328173
CAACGCA33400.042.4849555
AACGCAG34750.040.834466
TATCAAC41550.034.72732
TAGGTAT5250.032.2194335
ACGCAGA44550.031.8517957
CGCAGAG45150.031.5325878
AGGTATA5300.031.0289366
CTTAGGT5550.029.6312353
TTAGGTA5650.028.2751664
GCAGAGT52550.027.4498759
GTCTTAG8550.026.3863161
GTACATG57650.026.0072861
TACATGG59300.024.9666042
GGTATAG6400.024.961677
ACATGGG59200.024.604513
AGAGTAC45250.024.45383810-11
Produced by FastQC (version 0.11.2)