FastQCFastQC Report
Sun 14 Apr 2019
SRR6576076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309694
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17400.13285546089391873No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15190.11598129028612791No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15170.11582858285981307No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15120.11544681429402594No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14730.11246901948088638No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14420.11010205437300621No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14260.10888039496248741No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13200.10078690136780043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20300.063.6699561
GGTATCA6550.050.9465641
TCAACGC28050.045.7310874
ATCAACG28250.045.4073333
CAACGCA29500.043.3240135
AACGCAG30100.042.616526
TATCAAC36650.035.522512
ACGCAGA37450.034.2525257
CGCAGAG40650.031.440558
GCAGAGT45200.028.4835389
GTACATG52800.027.4166681
TACATGG52900.027.1871452
CCGTATA700.00883646126.8571432
ACATGGG54600.026.0754473
AGAGTAC38600.025.01533510-11
ATGGGAG12500.023.681675
TACGCAT1000.001761547623.493725
ATAGTAC1652.670502E-622.781793
GTCTTAG4450.022.1797751
CCCTATA1503.2918237E-521.9333342
Produced by FastQC (version 0.11.2)