FastQCFastQC Report
Sun 14 Apr 2019
SRR6576075_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576075_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461704
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17040.11657626988774744No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16080.11000859271097295No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15580.10658792751473622No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15240.10426187518129526No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14680.10043073016151012No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14630.10008866364188646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20500.062.7905731
TCAACGC28750.045.2869764
ATCAACG29750.043.6082153
GGTATCA7400.043.3274231
CAACGCA30250.042.8844875
AACGCAG31500.041.4811486
TATCAAC38050.034.3428542
ACGCAGA39600.032.8720477
CGCAGAG42800.030.5262178
GTACATG55800.028.814151
TACATGG56800.027.8059142
GCAGAGT47450.027.5347129
ACATGGG57850.027.219973
TAGGTAT2450.026.8582975
TAAGACA8350.025.3312954
ACCTAAG4500.025.1468941
AGAGTAC39950.024.82204810-11
CATGGGT14000.023.8375554
CTAAGAC7500.023.8159853
TATATAC2504.3655746E-1022.5625133
Produced by FastQC (version 0.11.2)