FastQCFastQC Report
Sun 14 Apr 2019
SRR6576075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461704
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17930.12266505393704881No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17540.11999693508398417No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17300.11835501578979055No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17130.11719198962307005No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16180.1106927257502203No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15210.10405663526952104No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT15200.10398822196559632No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15010.10268836919102636No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14980.10248312927925217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22000.058.345361
GGTATCA8650.053.8127821
TCAACGC31700.040.177534
ATCAACG32200.039.5536543
CAACGCA33200.038.5038385
AACGCAG34250.037.4606486
TATCAAC38550.033.1749952
GTACATG59650.030.3470631
TACATGG59400.029.9998532
ACGCAGA43800.029.0782577
ACATGGG61450.028.6037463
CGCAGAG45300.028.219158
GTCGTAT1205.8270016E-627.4273051
GTCTTAG6250.026.3302151
GCAGAGT49400.025.8770739
GTATAGG3700.024.14451
ACCTAAG4300.024.0558431
CCCTATA1602.0325642E-623.509122
CATGGGG36800.022.9878254
CGTATAG1050.002330537422.3896371
Produced by FastQC (version 0.11.2)