FastQCFastQC Report
Sun 14 Apr 2019
SRR6576073_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576073_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885319
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC24410.27571982528331596No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT19110.21585439824515232No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC18640.21054557735686233No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA18020.20354245192975637No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT16110.18196830746883327No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA14670.1657029838962001No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12930.1460490512459351No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC12260.13848115763922383No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC12030.13588322401303934No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA10700.12086039043553791No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC10690.12074743679961686No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10670.12052152952777473No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC10450.11803654953751133No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10420.11769768862974815No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT10260.11589043045501113No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC10000.11295363592106347No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT9280.10482097413474692No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT9190.10380439141145734No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9160.10346553050369414No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9040.10211008687264138No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT8930.1008675968775097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23450.052.2408941
CAACGCA29450.041.496555
ATCAACG30150.040.533123
GGTATCA9550.040.456711
GTACTAG5850.038.660321
TGTATAC651.4127277E-436.156023
ACGCAGA34650.035.536367
CGCAGAG35500.034.8198438
AACGCAG37050.034.2530676
TCAACGC36450.033.7852944
TATCAAC36800.033.7194182
GTTAGGC702.1838277E-433.5734443
GCAGAGT37650.032.581799
TAACCAC2300.030.6540165
TAGGCAT7950.030.1527545
TAGTACT8600.029.5134034
GGACTAA650.00615160828.9248166
TCAAGAC2350.028.0016823
TCTAACG3950.027.3687322
CTAGGCA9000.027.1571874
Produced by FastQC (version 0.11.2)