FastQCFastQC Report
Sun 14 Apr 2019
SRR6576067_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576067_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725254
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20690.28527936419516475No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT15840.2184062411237994No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14950.20613467833338386No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14870.20503161650952634No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT13020.17952331183281994No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC12630.17414588544151427No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11520.15884090263549047No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC10450.14408745074139542No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC10400.14339803710148447No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9980.1376069625262322No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9480.13071282612712237No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9320.1285067024794072No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9230.12726575792756745No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT8370.11540784332109853No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT8370.11540784332109853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.11209865784952583No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT8070.11127136148163265No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC7720.10644546600225577No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT7640.1053424041783982No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC7580.104515107810505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20600.052.099531
CAACGCA25200.040.6511575
ATCAACG25650.040.1211783
GGTATCA9400.038.5592841
TATCAAC29900.035.5233652
ACGCAGA29300.035.1231467
CTATTGA6350.034.8073169
GTACTAG5700.034.6849061
CGCAGAG30500.033.6126758
CCGAGTA702.176879E-433.590659
CTTAGGT702.186601E-433.5651783
AACGCAG32350.033.1190226
TCAACGC31650.033.1091544
GGGCTAT7350.032.606176
GCAGAGT31500.032.5456059
GGCTATT7150.031.546577
AGGGCTA7500.030.7009455
TAGGCAT7150.030.2321285
TAGTACT7000.030.2086584
GGGTACC3350.029.45727
Produced by FastQC (version 0.11.2)