FastQCFastQC Report
Sun 14 Apr 2019
SRR6576066_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576066_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794158
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC21350.26883819088896665No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT16810.21167072547276486No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC16020.20172308281223636No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14820.1866127395304209No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT13880.17477630395966545No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC13460.16948768381103005No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG12790.16105107547868308No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11990.1509775132908061No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA10410.1310822279697491No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC10300.129697113168916No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9860.12415665396558366No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9800.1234011368014929No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT9530.12000130956308441No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT8900.11206837934013131No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT8880.11181654028543438No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC8700.10954998879316206No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC8360.10526872486331434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.10123929998816356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.050.146291
GTACTAG6250.040.73081
CAACGCA25700.038.050615
ATCAACG26200.037.8627853
GACGTTA1401.2732926E-1136.9259767
ACGTTAA1451.8189894E-1135.6526648
TCTAACG3450.035.4311832
TATCAAC30700.033.3848572
ACGCAGA29850.032.590677
CTAACGC3900.032.548473
AACGCAG31550.032.334376
TAGGCAT8500.032.0804525
CGGGCGT6100.031.597896
CGCAGAG31300.031.381188
GGCGTCG6150.031.331138
GGTATCA9650.031.2652451
GCAGAGT31350.031.181229
CTAGGCA9100.030.9985434
GAGTTGC7900.030.93648194
CGCCTAC4050.030.1707177
Produced by FastQC (version 0.11.2)