FastQCFastQC Report
Sun 14 Apr 2019
SRR6576065_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576065_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919608
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15690.1706161755878592No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15450.1680063679306835No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12590.1369061600160068No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11860.12896799505876416No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT9370.10189124061556662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.059.1230281
GGTATCA6300.054.5523721
TCAACGC24250.046.895524
ATCAACG24800.045.8555033
CAACGCA25250.045.0382775
AACGCAG25750.044.3462376
TATCAAC29800.038.3214042
ACGCAGA32200.035.0254027
CGCAGAG34800.032.4314848
GCAGAGT35800.031.7882929
GTACATG39250.030.2268051
TACATGG40950.028.3461722
ACATGGG40350.027.9508783
CTTAGGT4050.026.687053
GTCTTAG4900.025.9417361
AGAGTAC32700.025.31044410-11
AGGTATA3950.024.9833096
CATGGGA19250.024.4116464
GGTAATC2002.2837412E-823.5128278
TTAGGTA4400.023.4962084
Produced by FastQC (version 0.11.2)