FastQCFastQC Report
Sun 14 Apr 2019
SRR6576064_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576064_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037482
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17300.16674988096179016No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15930.15354483258504725No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13070.12597808925841605No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12720.12260453675340874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21500.059.8009221
GGTATCA7600.052.6731151
TCAACGC28100.044.4959874
ATCAACG28450.043.7833633
CAACGCA29550.042.3125955
AACGCAG30650.040.94746
TATCAAC33600.037.492212
ACGCAGA37600.033.2488447
CGCAGAG39150.031.9324788
GTCTTAG7050.030.0612531
GCAGAGT42450.029.6715229
ACCTAAG6250.029.3878821
TACATCG804.7774942E-429.3782235
CTAAGAC7750.027.2933183
AGGTATA5300.026.6066936
GTACATG46000.026.312311
GGTATAG5500.025.6354717
ACATGGG47150.025.3219723
TACATGG47100.025.1492562
TAGGTAT5450.025.0119195
Produced by FastQC (version 0.11.2)