FastQCFastQC Report
Sun 14 Apr 2019
SRR6576059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307060
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9760.3178531883019605No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5810.18921383442975315No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5590.18204911092294668No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5090.16576564840747737No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4530.14752817039015176No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4260.13873510063179834No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4220.1374324236305608No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3910.12733667687096986No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3800.1237543151175666No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3680.11984628411385397No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3640.11854360711261643No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3620.11789226861199766No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3500.11398423760828502No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3450.1123558913567381No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3450.1123558913567381No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3430.11170455285611931No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.10681951410147854No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3280.10681951410147854No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3270.10649384485116917No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3260.10616817560085975No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3120.10160880609652836No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG3110.10128313684621898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATA250.005232396556.3999984
TATAGGT450.001012793741.777784
TAATAGT608.808979E-539.1666684
CCATTAG608.808979E-539.1666681
GTGTAGA853.9430415E-738.705881
TTAATCC500.001700085637.64
AGTATAC550.00271392334.1818163
AACGTGC600.004156278431.33333494
CGTAGAA300.004159487331.33333452-53
TATAGGA753.2699967E-431.3333344
TAATGCT600.004156278431.3333349
GTACATG21750.031.1172431
GGTACCT3650.030.9041088
TACATGG21750.030.9011522
ACATGGG21600.030.4629633
ATGGGTG1551.5097612E-930.3225825
TCTTAGG1452.6468115E-829.1724152
ATGGGTA4050.029.0123465
GGGGTAC650.00614724128.9230777
CATGGGT6250.028.5764
Produced by FastQC (version 0.11.2)