FastQCFastQC Report
Sun 14 Apr 2019
SRR6576058_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576058_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704967
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15090.21405257267361452No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11760.16681631906174332No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11400.16170969704964913No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10070.14284356572719006No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9770.13858804738377825No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9600.1361765869891782No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8790.1246866874619663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.11589194955224855No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8000.11348048915764851No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7930.11248753487751909No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7750.109934223871472No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7490.10624610797384842No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7270.1031253945220131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.065.103531
GTATCAA19650.064.4269261
ATCAACG29700.042.4041823
CAACGCA30100.042.309045
TCAACGC30050.041.9102864
AACGCAG31150.040.7320336
TATCAAC31400.040.2637862
CGATTCG250.00170345837.5941686-87
CTAACGC906.193277E-736.5498733
TCTAACG651.4130479E-436.1533552
CGCAGAG37350.033.994738
ACGCAGA37400.033.925217
GGTAATC1755.456968E-1232.2464338
GCAGAGT40000.031.5074569
TAATACC1052.066372E-631.3284654
GTACATG47000.029.1388761
ACATGGG46550.028.7710383
CTTAGGT3300.028.4804233
TACATGG48050.028.4637132
TAGGTAT3250.027.4726525
Produced by FastQC (version 0.11.2)